故事发生在江户时代末期。明石藩江户家老间宫在老中土井的府邸门前切腹自杀。间宫之死是一种抗议，抗议生性残忍的明石藩主松平齐昭不断杀害无辜百姓的暴行。齐昭是将军德川家庆的弟弟，明年将就任老中。这一自杀事件震动了幕府。 土井认为此事关乎国之存亡，下令御目付岛田新左卫门暗杀齐昭。 新左卫门领命，他四处奔走，召集到了十一名武艺高强的刺客，其中包括：御徒目付组长仓永、浪人剑客平山、新左卫门那个眠花宿柳嗜酒好赌的侄子新六郎。 暗杀计划虽是秘密筹备，却依然被齐昭的心腹鬼头半兵卫听到了风声。新左卫门与鬼头本是师兄弟，但两人选择了不同的道路。鬼头开始暗中布置与昔日同门展开较量。 新左卫门认为只有在从江户回明石的路上才能对齐昭发动奇袭，行动地点定在了落合宿。只要明石藩一行无法进入尾张藩，他们就会经过落合宿。为了能让齐昭入套，新左卫门将计划告诉了尾张藩的牧野靭负，请求他出手帮忙。牧野的儿子与儿媳被齐昭所杀，与之有旧仇。 刺客们迅速赶赴落合宿，要在那里建起足以迎击明石藩一行的要塞。途中，山民木贺小弥太也加入进来，连同新左卫门，共十三名刺客集结在了落合宿。 不料，众人左等右等，总不见齐昭来到落合宿。就在大家以为计划失败之时，两百多骑人马杀气腾腾而来。原来鬼头储备了足够兵力，准备诛杀新左卫门等人。混乱之中，大桥被炸，明石藩一行失去了退路。13人对300人的生死决战拉开了帷幕……
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